Abstract

The Emergence of drug-resistant strains of malaria parasites poses a threat to the worldwide malaria control efforts and it is very important to know the extent of these drug-resistant mutations regionally so as to determine the appropriate methods of treatment.

Chloroquine (CQ) had been used extensively in Cameroon, for decades leading up to 2002, its decreasing clinical effectiveness due to resistance, led the health authorities to resort to Amodiaquine (AQ) monotherapy in 2002 and then artemisinin-based combination therapy (ACT) in 2004 as the first-line treatment of uncomplicated malaria.

But despite the numerous efforts made by the WHO and the government of Cameroon to combat malaria, malaria still persists and the emergence of resistance to the ACTs which is the current first-line treatment for uncomplicated and multidrug-resistant malaria creates a growing need for the development of new drugs or the possible reintroduction of abandoned drugs like Chloroquine, which is a very safe, efficient and inexpensive therapy for malaria.

To assess the re-emergence of chloroquine-sensitive P. falciparum in Douala Bafoussam, Bafia, and the Mount Cameroon Area, 25 µL of blood was collected from malaria patients, on 3mm Whatman filter papers, P. falciparum positive Blood samples, were Amplified using Nested PCR.

Specific DNA sequences were amplified and Allele-specific Restriction analysis (RFLP) was performed on the PCR products, the fragments were Analyzed with 2% agarose gel and the Data was Analyzed with SPSS.

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