IMPROVING FIELD MAINTENANCE AND LAB CONDITIONS TO HELP ENGORGED CULICOIDES MILNEI SURVIVE BETTER, WHICH WILL BOOST THE LARGE-SCALE PRODUCTION OF INFECTIVE LARVAE OF MANSONELLA PERSTANS
Abstract
Culicoides are tiny blood sucking flies of the family ceratopogonidae, and are most abundant of hematophagous insect with over 1500 species approximaly known worldwide. Culicoides milnei is the principal vector for the transmission of Mansonella perstans, which is one of the filarial nematodes causing Masonellosis in the human population. There have been little or knowledge in either maintaining this wild species (Culicoides milnei) in captivity in the field or rearing them in the laboratory.
This work was designed to optimized Field maintenance and Laboratory Rearing Conditions for the Improvement of Survival of Engorged Culicoides milnei for the Large Scale Production of Infective larvae of Mansonella perstans.
A total of 6100 culicoides milnei were collected from 2 donors with microfilaria load of (16450mf/ml) (750mf/ml) respectively. The engorged flies were fed daily with 15% sucrose solution while mortality and survival was visually monitor under different rearing conditions until dissection.
Data was exported in to SPSS in to excel sheet for analysis. Kruskal wallis test and Mann whitney test was used to test for different variable. It was observed that flies maintain and reared up 12days had the highest survival (79.1%) with a significant difference (P < 0.001). as compare to 13 and 14days.
Flies that were reared from donor 2 had a survival rate of (77.8%) with a significant difference of (P < 0.001) For the various rearing conditions, flies maintain and reared inside cold box (on ice park) had a survival rate of (70.1%) with a significant different of (P = 0.0143). Flies in which tubes were moist with distilled had (69.1%) survival with a significant difference of (P = 0.0146).
Lastly, flies maintained and reared under the temperature 24ᵒC/75% RH had (71.8%) survival with a significant difference (P < 0.001). For the standard conditions to maintain and reared the engorged flies to survive for the large scale production of infective larvae in the field and laboratory the flies, should be place inside cold (ice park), tubes moist with distilled water and reared under temperature of 24ᵒC/75%RH.
Check out: Microbiology Project Topics with Materials
| Project Details | |
| Department | Microbiology |
| Project ID | MCB0028 |
| Price | Cameroonian: 5000 Frs |
| International: $15 | |
| No of pages | 75 |
| Methodology | Descriptive |
| Reference | yes |
| Format | MS word & PDF |
| Chapters | 1-5 |
| Extra Content | table of content, questionnaire |
This is a premium project material, to get the complete research project make payment of 5,000FRS (for Cameroonian base clients) and $15 for international base clients. See details on payment page
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IMPROVING FIELD MAINTENANCE AND LAB CONDITIONS TO HELP ENGORGED CULICOIDES MILNEI SURVIVE BETTER, WHICH WILL BOOST THE LARGE-SCALE PRODUCTION OF INFECTIVE LARVAE OF MANSONELLA PERSTANS
| Project Details | |
| Department | Microbiology |
| Project ID | MCB0028 |
| Price | Cameroonian: 5000 Frs |
| International: $15 | |
| No of pages | 75 |
| Methodology | Descriptive |
| Reference | yes |
| Format | MS word & PDF |
| Chapters | 1-5 |
| Extra Content | table of content, questionnaire |
Abstract
Culicoides are tiny blood sucking flies of the family ceratopogonidae, and are most abundant of hematophagous insect with over 1500 species approximaly known worldwide. Culicoides milnei is the principal vector for the transmission of Mansonella perstans, which is one of the filarial nematodes causing Masonellosis in the human population. There have been little or knowledge in either maintaining this wild species (Culicoides milnei) in captivity in the field or rearing them in the laboratory.
This work was designed to optimized Field maintenance and Laboratory Rearing Conditions for the Improvement of Survival of Engorged Culicoides milnei for the Large Scale Production of Infective larvae of Mansonella perstans.
A total of 6100 culicoides milnei were collected from 2 donors with microfilaria load of (16450mf/ml) (750mf/ml) respectively. The engorged flies were fed daily with 15% sucrose solution while mortality and survival was visually monitor under different rearing conditions until dissection.
Data was exported in to SPSS in to excel sheet for analysis. Kruskal wallis test and Mann whitney test was used to test for different variable. It was observed that flies maintain and reared up 12days had the highest survival (79.1%) with a significant difference (P < 0.001). as compare to 13 and 14days.
Flies that were reared from donor 2 had a survival rate of (77.8%) with a significant difference of (P < 0.001) For the various rearing conditions, flies maintain and reared inside cold box (on ice park) had a survival rate of (70.1%) with a significant different of (P = 0.0143). Flies in which tubes were moist with distilled had (69.1%) survival with a significant difference of (P = 0.0146).
Lastly, flies maintained and reared under the temperature 24ᵒC/75% RH had (71.8%) survival with a significant difference (P < 0.001). For the standard conditions to maintain and reared the engorged flies to survive for the large scale production of infective larvae in the field and laboratory the flies, should be place inside cold (ice park), tubes moist with distilled water and reared under temperature of 24ᵒC/75%RH.
Check out: Microbiology Project Topics with Materials
This is a premium project material, to get the complete research project make payment of 5,000FRS (for Cameroonian base clients) and $15 for international base clients. See details on payment page
NB: It’s advisable to contact us before making any form of payment
Our Fair use policy
Using our service is LEGAL and IS NOT prohibited by any university/college policies. For more details click here
We’ve been providing support to students, helping them make the most out of their academics, since 2014. The custom academic work that we provide is a powerful tool that will facilitate and boost your coursework, grades and examination results. Professionalism is at the core of our dealings with clients
For more project materials and info!
Contact us here
OR
Click on the WhatsApp Button at the bottom left
Email: info@project-house.net
