The use of amylase enzyme in the manufacturing of a number of goods is widespread around the world, including Cameroon.

The lack of commercial amylase production in the nation, however, is a serious issue for these companies. Amylases are a type of industrial enzyme that is commonly employed. They’re used in the food, pharmaceutical, and textile sectors, as well as clinical diagnostics.

Most industrial operations take place at extremely high temperatures, necessitating the usage of thermostable enzymes. The majority of commercially available enzymes are not thermostable, and hence will have a detrimental impact on output and yield if utilized in an industrial process.

Because enzymes that aren’t thermostable become inactive at high temperatures, this is the case. The firm suffers loses if this occurs in the middle of an industrial operation.

Thermostable amylases have the following advantages over other enzymes: easy use in industrial processing with good yield; they can be easily immobilized and reused because they do not inactivate at high temperatures due to their thermostability; and they are stable to high temperatures, reducing the risk of contamination.

In order to satisfy the demands of all starch processing businesses, it is necessary to increase amylase enzyme production and maximize yield.

Because of the growing demand for amylase enzyme, it is necessary to increase production and improve output in order to fulfill the demands of its many uses.

The OVAT (One Variance at a Time) technique is the most popular method of optimization. The OVAT method is time-consuming and prone to errors. Because enzymes are in such great demand, they require precise and time-saving manufacturing processes.

Studying the optimization of circumstances for a higher amylase yield might lead to the development of a technique that will aid in the rapid manufacturing of thermostable amylases on a big scale.

Cameroon has a diverse range of industries, which necessitates the usage of thermostable amylases. Unfortunately, these enzymes are in short supply in the nation.

Despite Cameroon’s biodiversity, which can lead to the production of amylase, little or no research has been done on the development and optimization of thermostable amylases.

For industrial microbes, soil is one of the most fertile ecosystems. As a result, there’s a considerable chance that bacteria in the soil can create amylase that’s thermostable and hence suitable for industrial procedures.

Hypothesis

The soils in Buea contain bacteria capable of producing thermostable amylases which can be produced in large quantities under laboratory conditions.

Objectives

General Objective

To study the factors that can help in the optimization of production of thermostable amylases by bacteria isolated from soil in Buea

Specific Objectives

The specific objectives of this study were:

1. Isolation and characterization of thermostable amylase-producing bacteria.
2. To use a factorial design to study the factors that influence thermostable amylase production (Effect of temperature, pH, incubation period, minerals, and different carbon sources).
3. Optimization of thermostable amylase production using central composite design.